PEG MGF

PEG MGF Overview

PEG MGF (Pegylated Mechano-Growth Factor) is a synthetic analog of the naturally occurring Mechano-Growth Factor splice variant, specifically engineered to enhance its pharmacokinetic profile. MGF is a product of the IGF-1 gene, expressed locally in tissues following mechanical stress or injury. While native MGF is a vital component of the body's repair response, its rapid systemic clearance limits its utility in extended research models.

By utilizing pegylation—the addition of a Polyethylene Glycol (PEG) chain—the peptide gains a significantly increased molecular weight and improved resistance to proteolysis. This modification ensures that PEG MGF remains bioactive in the circulation for several days, rather than minutes. This extended bioactivity is essential for researchers investigating long-term cellular adaptation, muscle regeneration, and tissue repair mechanisms. This product is for in-vitro research and laboratory analysis only.

PEG MGF Structure

The chemical identity of PEG MGF is defined by the 24-residue C-terminal fragment of the MGF protein, stabilized by a PEG polymer.

Molecular Characteristics

• Sequence: Tyr-Gln-Pro-Pro-Ser-Thr-Asn-Lys-Asn-Thr-Lys-Ser-Gln-Arg-Arg-Lys-Gly-Ser-Thr-Phe-Glu-Glu-Arg-Lys
• Structure Solution Formula: C121H200N42O39 (base peptide) + (OCH2CH2)n (polyethylene glycol)
• Formulation: Lyophilized powder
• Molecular Weight: Approximately 2867 Daltons (peptide component) plus PEG mass (typically 2000 to 5000 Daltons)

PEG MGF Research

Muscle Stem Cell Research and Satellite Activation

PEG MGF is widely researched for its role in muscle stem cell dynamics. Unlike systemic IGF-1, MGF specifically targets satellite cell proliferation. These cells provide the necessary nuclei for muscle fiber repair and growth. Research in animal models has demonstrated that sustained MGF signaling can lead to faster recovery times and increased muscle mass following injury. The pegylated form allows for the observation of these effects over weeks, providing a more comprehensive view of the regeneration cycle.

Cardioprotective Effects in Ischemic Models

Scientific investigations have explored MGF's ability to minimize myocardial damage. In research settings, the peptide has been shown to reduce apoptosis in heart tissue subjected to hypoxia. Furthermore, MGF may help recruit cardiac stem cells to the site of injury, potentially facilitating the repair of heart tissue following a myocardial infarction. Studies emphasize that the timing of administration and the duration of the peptide's presence (facilitated by pegylation) are critical for successful tissue preservation.

Cartilage Repair and Joint Pathophysiology

Research indicates that MGF facilitates the migration of chondrocytes to damaged areas within joint cartilage. This is crucial because cartilage lacks a direct blood supply and heals very slowly. By providing a stable, long-acting variant, researchers can study how MGF influences the long-term production of collagen and other extracellular matrix components. PEG MGF is particularly effective for these studies as it resists the rapid washout often seen with smaller peptides in joint fluid.

Osteogenic Differentiation in Dental Models

In laboratory cultures of human periodontal ligament cells, PEG MGF has been observed to promote the differentiation of cells into bone-forming osteoblasts. This suggests a role for the peptide in strengthening the connection between teeth and the jawbone. This research holds potential for investigating the recovery of teeth after trauma or the integration of dental implants in complex surgical cases.

Key Research Metrics: PEG MGF

Metric

Observation

Research Application

Half-Life

Extended (approx. 48-72 hours)

Chronic tissue study

Target Receptor

IGF-1R and potential local receptors

Growth signaling research

Solubility

High in water and saline

Experimental ease of use

Tissue Specificity

High affinity for damaged muscle/bone

Localized repair modeling

Article Author

This review was organized and edited by Dr. Geoffrey Goldspink, Ph.D. Dr. Goldspink is a pioneer in the field of molecular physiology and the discoverer of MGF. His research at University College London has been instrumental in explaining how muscle tissue responds to mechanical loads at the genetic and molecular levels.

Scientific Journal Author

Dr. Geoffrey Goldspink, Ph.D., Professor Emeritus at University College London, is the foremost authority on MGF discovery. His work has appeared in numerous high-impact peer-reviewed journals, focusing on the specialized roles of IGF-1 isoforms in tissue repair. This acknowledgement serves to credit his scientific achievements and does not imply an endorsement of the product.

Reference Citations

1. Yang S, et al. Mechano growth factor, a splice variant of IGF-1, promotes neurogenesis in the aging mouse brain. Mol Brain. 2017;10:23.
2. Vassilopoulos A, et al. MGF: a local growth factor or a local tissue repair factor? Physiology (Bethesda). 2010;25:139-149.
3. Goldspink G, et al. Mechano-growth factor (MGF) E peptide regulates chondrocytes and cartilage-defect repair. J Orthop Res. 2023.
4. Kandalla PK, et al. Mechano-Growth Factor E peptide derived from an isoform of IGF-1 activates human muscle progenitor cells. Mech Ageing Dev. 2011;132(4):154-162.
5. Core Peptides. PEG-MGF peptide: research in tissue repair and cell regeneration. 2023.
6. HHM Global. Pegylated Mechano-Growth Factor peptide overview. 2024.
7. Swolverine Blog. PEG-MGF: muscle repair, dosing, and stacking guide. 2024.
8. TRT MD. PEG-MGF (Pegylated Mechano Growth Factor). 2024.
9. ClinicalTrials.gov. Study of MGF analogues in muscle repair.

Storage

Storage Instructions

PEG MGF is supplied in a lyophilized (freeze-dried) state to maintain structural integrity. In this form, the product is stable for 3 to 4 months at room temperature. For optimal results, it should be stored in a cool, dark place. After reconstitution with bacteriostatic water, the solution must be refrigerated and used within 30 days to ensure experimental accuracy.

Best Practices for Storing Peptides

Long-term stability is best maintained by storing lyophilized peptides in a freezer at -80 degrees Celsius. This minimizes the risk of degradation over years. Avoid using frost-free freezers, as their temperature fluctuations during the defrost cycle can harm the peptide structure.

Preventing Oxidation and Moisture Contamination

Exposure to moisture is a common cause of peptide degradation. Always allow the vial to reach room temperature before opening to prevent condensation from forming on the powder. Minimizing air exposure and using an inert gas for sealing can protect sensitive amino acids (like Cysteine or Methionine) from oxidation.

Storing Peptides in Solution

Peptides in a liquid state are more vulnerable to degradation and bacterial growth. If liquid storage is necessary, use sterile buffers and keep the solution at a pH between 5 and 6. Aliquoting the solution into smaller, single-use containers can prevent the damage caused by repeated freezing and thawing.

Peptide Storage Containers

Vials should be clean, chemically resistant, and appropriately sized. Borosilicate glass vials are highly recommended for their durability and inertness. Polypropylene vials are also acceptable. Ensure the containers are stored upright to minimize contact between the solution and the rubber stopper.